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Double (fluorescent and spin) sensors for detection of reactive oxygen species in the thylakoid membrane
1Institute of Organic and Medicinal Chemistry, University of Pécs, Hungary.
Free Radical Biology & Medicine
|April 29, 1998
Abstract:
A series of dansylated sterically hindered amines designed to trapping reactive oxygen species, were synthesized. Compounds were tested in isolated thylakoid membranes subjected to photoinhibition by excess photosynthetically active radiation (400-700 nm). DanePy showed good selectivity for singlet oxygen and the formation of nitroxide was detected by appearance of ESR signal and quenching fluorescence.