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Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
Published on: January 7, 2020
Disruption and sequence identification of 2,000 genes in mouse embryonic stem cells
B P Zambrowicz1, G A Friedrich, E C Buxton
1Lexicon Genetics, The Woodlands, Texas 77381, USA. materials@lexgen.com
Nature
|April 29, 1998
Summary
Researchers developed a high-throughput gene trapping method to address the gene function gap. This technique enables automated identification of mutated genes, aiding large-scale functional genomics in mammals.
Area of Science:
- Genomics
- Mammalian genetics
- Functional genomics
Background:
- The rapid growth of genomic data has outpaced functional gene identification, creating a 'gene function gap'.
- Large-scale mutagenesis in embryonic stem (ES) cells is crucial for determining mammalian gene function.
Purpose of the Study:
- To develop a high-throughput gene trapping method for large-scale gene function studies.
- To create a comprehensive library of sequence-tagged mutations in mouse ES cells.
Main Methods:
- Utilized a gene trapping approach for high-throughput mutagenesis in ES cells.
- Developed automated identification of sequence tags from mutated genes.
- Applied the method to create the Omnibank ES cell library.
Main Results:
- Successfully trapped and mutated genes irrespective of their expression status in ES cells.
- Generated sequence-tagged mutations for approximately 2,000 genes.
- Established a scalable method for mammalian gene function discovery.
Conclusions:
- The described gene trapping method effectively bridges the gene function gap.
- Omnibank provides a valuable resource for large-scale functional genomics research.
- This approach facilitates systematic gene function analysis in mammals.
