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Related Experiment Videos

Multiple endosomal recycling pathways in rat adipose cells

K V Kandror1, P F Pilch

  • 1Department of Biochemistry, Boston University School of Medicine, Boston, MA 02118, USA.

The Biochemical Journal
|April 30, 1998
PubMed
Summary

Insulin prompts fat cells to move glucose transporter Glut4 to the surface. This study reveals Glut4 shares an intracellular pathway with the transferrin receptor, distinct from the insulin receptor pathway.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Endocrinology

Background:

  • Adipose and skeletal-muscle cells translocate membrane proteins to the cell surface in response to insulin.
  • Glut4 (glucose transporter type 4) is key for insulin-mediated glucose clearance.
  • The intracellular localization and trafficking pathway of Glut4 remain unclear.

Purpose of the Study:

  • To elucidate the intracellular compartment and trafficking pathway of Glut4.
  • To investigate the co-localization and co-trafficking of Glut4 with other membrane proteins.

Main Methods:

  • Subcellular fractionation
  • Cell-surface biotinylation
  • Radioactive-ligand uptake (125I-transferrin)

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Main Results:

  • The Glut4-containing intracellular compartment also houses the transferrin receptor, aminopeptidase gp160 (IRAP), sortilin, and IGF-II/MPR.
  • Transferrin receptor recycles between this compartment and the plasma membrane with Glut4 upon insulin stimulation.
  • The insulin receptor traffics independently from Glut4, utilizing a separate pathway.

Conclusions:

  • The 'Glut4 pathway' likely originates from hormone-insensitive endosomes.
  • Glut4 is specifically targeted to the transferrin receptor pathway in adipose cells.
  • Insulin receptor and Glut4 follow distinct intracellular trafficking routes.