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Preparation of the Rat Vocal Fold for Neuromuscular Analyses
Published on: May 15, 2020
Edema formation in the rat larynx
M Lidegran1, S Domeij, B Carlsöö
1Department of Otorhinolaryngology, Huddinge University Hospital, Karolinska Institute, Stockholm, Sweden.
Cell and Tissue Research
|June 2, 1998
Summary
Compound 48/80 triggers mast cell degranulation and laryngeal edema in rats. Substance P and capsaicin increase vascular permeability via a different mechanism, distinct from mast cell activation.
Area of Science:
- Laryngology
- Cell Biology
- Pharmacology
Background:
- Mast cells play a crucial role in inflammatory responses within the larynx.
- Understanding the mechanisms of laryngeal edema is vital for treating airway inflammation.
Purpose of the Study:
- To investigate the effects of compound 48/80, substance P, and capsaicin on laryngeal plasma exudation and edema formation in rats.
- To differentiate the mechanisms of action of these agents on mast cells and vascular permeability.
Main Methods:
- Light and electron microscopy were used to examine laryngeal tissues.
- Rats received intravenous injections of compound 48/80, substance P, or capsaicin.
- Morphological changes in connective tissue mast cells and laryngeal tissues were analyzed.
Main Results:
- Compound 48/80 induced mast cell degranulation and significant edema in the subglottic region and epiglottis.
- Substance P and capsaicin caused plasma exudation and widened intercellular spaces but did not degranulate mast cells.
- Edema induced by compound 48/80 was attributed to histamine and serotonin release from mast cells.
Conclusions:
- Compound 48/80 exerts its effects through mast cell degranulation and mediator release.
- Substance P and capsaicin increase vascular permeability via a direct action on blood vessels, independent of mast cell degranulation.
