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Related Experiment Videos

Apoptosis dependent decrease of the intramembrane ion traffic in cultured mouse fibroblasts shown by conductivity

A Bonincontro1, A Iacoangeli, G Melucci-Vigo

  • 1Dipartimento di Genetica e Biologia Molecolare, Università di Roma La Sapienza.

Bioscience Reports
|April 30, 1998
PubMed
Summary
This summary is machine-generated.

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Programmed cell death, or apoptosis, significantly reduces ion transport across cell membranes. This study reveals decreased membrane conductivity in apoptotic cells, suggesting impaired plasma membrane function is an early event in cell death.

Area of Science:

  • Cell Biology
  • Biophysics
  • Biochemistry

Background:

  • Apoptosis is a crucial biological process involving programmed cell death.
  • Understanding the early molecular events in apoptosis is vital for various research fields.
  • Plasma membrane function changes during cellular stress and death pathways.

Purpose of the Study:

  • To investigate alterations in intramembranal ion traffic during apoptosis in 3T6 cells.
  • To determine if reduced plasma membrane function is an early indicator of apoptosis.
  • To explore the role of membrane conductivity changes in programmed cell death.

Main Methods:

  • Apoptosis was induced in 3T6 cells using serum deprivation, high density, and hydrogen peroxide.
  • Cell death was confirmed via DNA fragmentation, electrophoresis, immunofluorescence, and histology.

Related Experiment Videos

  • Electrical conductivity of cell suspensions was measured across varying electrical field frequencies to assess membrane properties.

    • Main Results:

    • Apoptosis induction led to a measurable decrease in cell membrane conductivity.
    • This reduction in conductivity indicates diminished intramembranal ion traffic in apoptotic cells.
    • The findings suggest a significant impairment of plasma membrane function during early apoptosis.

    Conclusions:

    • Reduced plasma membrane function and ion permeability are early events in apoptosis.
    • These membrane alterations occur independently of nuclear-specific events.
    • The study highlights the plasma membrane's role in the apoptotic cascade, not just the nucleus.