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Published on: June 19, 2015
Characterization of new insertion-like sequences of Enterococcus hirae and their dissemination among clinical
L A Sechi1, R Franklin, I Duprè
1Department of Microbiology and immunology, Temple University School of Medicine, Philadelphia, PA 19140, USA. sardinia@mailexcite.com
Abstract:
Sequence analysis of different fragments that hybridized with a 4.5-kb EcoRI fragment originally cloned from Enterococcus hirae ATCC 9790 showed 66% homology to IS-like sequences found in staphylococci and lactococci. We tested several enterococcal ATCC strains and found that only E. hirae ATCC 9790 and Enterococcus faecium ATCC 19434 hybridized with the IS-like sequence. Moreover, we wanted to investigate the dissemination of this new IS among E. faecium strains. We analyzed 131 clinical E. faecium isolated in Italy and the USA for the presence of the IS and we found its presence in more than 63% of the isolates. The hybridization patterns obtained vary considerably between unrelated strains and allow further classification among ribotype-grouped species.
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