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Arachidonic acid and human bone marrow stromal cells
Y Denizot1, C Dulery, F Trimoreau
1Laboratoire d'Hématologie Expérimentale, Faculté de Médecine, Limoges, France.
Biochimica Et Biophysica Acta
|April 30, 1998
Summary
Human bone marrow stromal cells capture and utilize arachidonic acid (AA). These cells release AA metabolites, prostaglandin E2 (PGE2) and leukotriene B4 (LTB4), influencing hematopoietic progenitor growth.
Area of Science:
- Hematology
- Cell Biology
- Biochemistry
Background:
- Human bone marrow stromal cells regulate hematopoietic progenitor growth via cytokines.
- Arachidonic acid (AA) is a precursor to prostaglandin E2 (PGE2) and leukotriene B4 (LTB4), which modulate progenitor growth.
Purpose of the Study:
- Investigate AA incorporation in bone marrow stromal cells.
- Analyze PGE2 and LTB4 production by these cells.
- Determine the effect of AA on stromal cell growth.
Main Methods:
- Gas chromatography to detect AA in plasma and stromal cell cultures.
- [3H]-AA incorporation and prelabeling-chase experiments in stromal cells.
- Stimulation of stromal cells with phorbol myristic acetate (PMA) and tumor necrosis factor alpha (TNF-alpha) to measure metabolite release.
Main Results:
- AA is present in bone marrow plasma and stromal cells, incorporated into triglycerides and phospholipids (preferentially phosphatidylethanolamine).
- Stromal cells release PGE2 and LTB4 upon stimulation with PMA and TNF-alpha.
- Exogenous AA did not significantly affect stromal cell growth.
Conclusions:
- Human bone marrow stromal cells capture exogenous AA, potentially controlling marrow AA levels.
- These cells regulate hematopoiesis through the secretion of AA metabolites like PGE2 and LTB4.