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Microbiological investigations to validate the preparation of corneal transplants
1Department of Hygiene, University of Kiel, Germany.
Abstract:
The globe and cornea are colonized by microorganisms and cannot be removed as sterile products. Antiseptic measures and storage of the cornea in medium containing antibiotics lead to an obvious reduction of the microbial flora. Unfortunately there is the possibility of secondary microbial contamination during the preparation. Large amounts of bacteria or resistant bacteria can ruin attempts to reduce the microbial flora. To validate the whole process of preparation we investigated the contamination of removed globes, environment, materials, instruments and media for each sequence of operation and eliminated sources of contamination. Recommendations are given to ensure a virtually sterile product at the end of preparation.
Insights
Corneal preparation involves microbial contamination risks. This study identified and eliminated contamination sources, providing recommendations for achieving a virtually sterile corneal product for transplantation.
Area of Science:
- Ophthalmology
- Microbiology
- Surgical Preparation
Background:
- The cornea, globe, and ocular environment are naturally colonized by microorganisms.
- Achieving sterile corneal grafts is challenging due to potential secondary contamination during preparation.
- High bacterial loads or resistant strains can compromise microbial reduction efforts.
Purpose of the Study:
- To investigate and identify sources of microbial contamination throughout the corneal preparation process.
- To validate the entire preparation workflow for microbial safety.
- To develop recommendations for producing a virtually sterile corneal product.
Main Methods:
- Systematic monitoring of microbial contamination on removed globes, surgical environment, materials, instruments, and media.
- Sequential analysis of contamination at each operational step.
- Identification and elimination of identified contamination sources.
Main Results:
- Specific sources of microbial contamination were identified at various stages of corneal preparation.
- The implemented interventions effectively reduced microbial contamination.
- The study established a validated process for minimizing microbial load.
Conclusions:
- Thorough investigation and control of contamination sources are crucial for sterile corneal preparation.
- Recommendations derived from this study can enhance the safety of corneal transplantation.
- A virtually sterile corneal product can be achieved through meticulous process validation and control.