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The role of tissue mast cells in polyacrylamide gel-induced inflammation in mice
R R Harris1, D Wilcox, R L Bell
1Immunoscience Research, Abbott Laboratories, Abbott Park, IL 60064-3500, USA. Richard.R.Harris@abbott.com
Objective And Design:
In the present study, we investigated the role of mast cells in a model of polyacrylamide gel (PAG)-induced inflammation in mice.
Subjects:
Balb/c mice and two strains of mast cell deficient mice (WBB6F1/J-W/Wv, WCB6F1/J-S1/S1d).
Treatment:
Various quantities of polyacrylamide gel (Bio-Gel P4) were injected subcutaneously in the backs of mice.
Methods:
Five hours after the injection of PAG the animals were euthanized, the injection sites lavaged and levels of LTB4, PGE2, TNF alpha and cells were determined.
Results:
Subcutaneous injection of PAG caused a time-dependent response characterized by the accumulation of inflammatory cells peaking at 10 h and the formation of LTB4, PGE2 and TNF alpha, peaking at 5 h. PAG injection into W/Wv or SL/SLd mice (mice lacking mast cells) resulted in an attenuated response, i.e. LTB4 levels were reduced by 60% and minimal cell influx was seen. The lack of mast cells caused about a 30% reduction in the levels of TNF alpha found.
Conclusions:
These data suggest that mast cells play a prominent role in the PMN influx, TNF alpha production and eicosanoid formation in the PAG-induced inflammatory response.