Related Experiment Videos
Characterization of erythroid cell-derived natural suppressor activity
V I Seledtsov1, G V Seledtsova, D M Samarin
1Department of Experimental Immunology, Institute of Clinical Immunology, Novosibirsk, Russia.
Immunobiology
|May 1, 1998
Summary
Nucleated erythroid cells (NEC) directly suppress B cell proliferation but not T cell responses. This suppression is partly mediated by soluble molecules, including transforming growth factor-beta (TGF-beta).
Area of Science:
- Immunology
- Cell Biology
Background:
- Nucleated erythroid cells (NEC) are known to suppress antibody-mediated immune responses.
- The precise mechanisms and targets of NEC-mediated suppression require further elucidation.
Purpose of the Study:
- To investigate the direct effects of NEC on B cell and T cell responses in vitro.
- To identify the soluble factors involved in NEC-mediated suppression.
Main Methods:
- Separation of NEC from phenylhydrazine-treated mouse spleens.
- In vitro culture of NEC with activated B cells and in mixed lymphocyte cultures (MLC).
- Assessment of B cell proliferation, T cell proliferation, cytotoxic T lymphocyte (CTL) generation, and interleukin-2 (IL-2) production.
- Use of antisense oligodeoxynucleotides (OD) and anti-TGF-beta antibodies (Ab) to block TGF-beta synthesis and activity.
Main Results:
- NEC directly suppressed the proliferative response of preactivated B cells to lipopolysaccharide (LPS).
- NEC did not significantly affect T cell proliferation, CTL generation, or IL-2 production in MLC or Con A-activated T cells.
- NEC-conditioned medium (CM) demonstrated suppressive activity on B cell blastogenesis.
- Blocking TGF-beta synthesis or neutralizing its activity diminished the suppressive capacity of NEC-CM on B cell blastogenesis.
Conclusions:
- NEC can directly suppress B cell responses without affecting T cell responses.
- NEC mediate their natural suppressor (NS) activity, at least in part, through the release of TGF-beta.