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Related Experiment Videos

Dbp5p, a cytosolic RNA helicase, is required for poly(A)+ RNA export

S S Tseng1, P L Weaver, Y Liu

  • 1Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210, USA.

The EMBO Journal
|June 20, 1998
PubMed
Summary

The DBP5 gene encodes an RNA helicase essential for messenger RNA export in yeast. This protein, Dbp5p, functions in the cytoplasm to facilitate the transport of polyadenylated RNA out of the nucleus.

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • The DBP5 gene in Saccharomyces cerevisiae encodes a putative RNA helicase.
  • The precise function of Dbp5p, an ATP-dependent RNA helicase, was previously unknown.
  • Understanding RNA helicase function is crucial for cellular processes like gene expression and transport.

Purpose of the Study:

  • To elucidate the function of the DBP5 gene product, Dbp5p, in yeast.
  • To investigate the role of Dbp5p in RNA metabolism and transport.
  • To determine the subcellular localization and functional significance of Dbp5p.

Main Methods:

  • Genetic analysis of the DBP5 gene in Saccharomyces cerevisiae.
  • Biochemical assays to characterize Dbp5p as an ATP-dependent RNA helicase.

Related Experiment Videos

  • Subcellular localization studies using microscopy to determine Dbp5p's distribution.

    • Main Results:

    • Dbp5p is an ATP-dependent RNA helicase.
    • Dbp5p is essential for the export of polyadenylated [poly(A)+] RNA.
    • Dbp5p is predominantly localized to the cytoplasm, enriched at the nuclear envelope.

    Conclusions:

    • Dbp5p plays a critical role in the nuclear export of polyadenylated RNA.
    • Its cytoplasmic localization suggests a role in remodeling messenger ribonucleoprotein particles (mRNPs) and coupling export with translation.
    • The conserved nature of DBP5 homologs indicates a potentially conserved function across eukaryotes.