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Related Experiment Videos

Allograft heart valve viability and valve-processing variables

K L Gall1, S E Smith, C A Willmette

  • 1Department of Cardiac Surgery, The Prince Charles Hospital, Chermside, Brisbane, Australia.

The Annals of Thoracic Surgery
|May 16, 1998
PubMed
Summary

Maximizing allograft pulmonary valve viability is crucial for durability. Cryopreservation, if not used within 1-2 days, and avoiding amphotericin B are key for preserving valve viability.

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Area of Science:

  • Cardiovascular Surgery
  • Tissue Engineering
  • Transplantation Immunology

Background:

  • The relationship between allograft valve viability and long-term durability is debated.
  • Previous clinical findings suggest viable cryopreserved valves outperform non-viable refrigerated valves.
  • This study evaluates valve processing protocols' impact on viability.

Purpose of the Study:

  • To quantitatively assess the effects of different valve-processing protocols on allograft pulmonary valve viability.
  • To determine the impact of donor type, antibiotics, and storage conditions on valve viability.

Main Methods:

  • Quantitative analysis of pulmonary valve viability using thin-layer autoradiography.
  • Assessment of viability following various processing steps including cryopreservation and antibiotic treatment.

Related Experiment Videos

  • Comparison of viability between beating-heart and non-beating-heart donors.

    • Main Results:

    • Beating-heart donors yielded higher initial valve viability (0.92+/-0.02) compared to non-beating-heart donors (0.66+/-0.03).
    • Cryopreservation reduced viability to 50%-60% of control; amphotericin B further decreased it to 10%-36%.
    • Storage at 4°C for 7 days reduced viability to 2%, and to 0% after 21 days.

    Conclusions:

    • For optimal preimplantation viability, procure valves promptly after cardiac arrest and cryopreserve if not implanted within 1-2 days.
    • Amphotericin B should be avoided during cryopreservation to maximize valve viability.
    • These findings inform strategies for improving allograft valve graft survival and durability.