Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Calcium preconditioning in human myocardium

B S Cain1, D R Meldrum, X Meng

  • 1Department of Surgery, University of Colorado Health Sciences Center, Denver 80262, USA.

The Annals of Thoracic Surgery
|May 16, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CT scanning of middle ear cholesteatoma: what does the surgeon want to know?

The British journal of radiology·2002
Same author

First crystallographic signature of amyloid-like fibril forming beta-sheet assemblage from a tripeptide with non-coded amino acids.

Chemical communications (Cambridge, England)·2002
Same author

Audit of stapedectomy results in a district general hospital.

Clinical otolaryngology and allied sciences·2002
Same author

Coronary artery disease and its problems in management.

Journal of the Indian Medical Association·2002
Same author

Leucopenia in adult polycystic kidney disease patients on haemodialysis.

Nephron·2002
Same author

Traditional healers and their practices in Malawi.

Tropical doctor·2002
Same journal

Does prior percutaneous coronary revascularization negatively affect the outcomes of subsequent coronary artery bypass grafting?

The Annals of thoracic surgery·2026
Same journal

Lymph Node Dissection and Chylothorax - Balancing Oncologic Benefit Against Morbidity.

The Annals of thoracic surgery·2026
Same journal

Preserved Antegrade Pulmonary Blood Flow in Bidirectional Glenn: Outcomes and Considerations for Staged Palliation.

The Annals of thoracic surgery·2026
Same journal

Domo Arigato, Mr. Roboto.

The Annals of thoracic surgery·2026
Same journal

Impact of High SUVmax on Recurrence by Resection Strategy in Stage IA Adenocarcinoma ≤2 cm.

The Annals of thoracic surgery·2026
Same journal

Preoperative Treatment for Stage II NSCLC Requires Multidimensional Consideration.

The Annals of thoracic surgery·2026
See all related articles

Calcium (Ca2+) preconditioning protects human heart tissue from damage caused by ischemia-reperfusion injury. This protective effect is dependent on protein kinase C (PKC) signaling pathways.

Area of Science:

  • Cardiology
  • Molecular Biology
  • Physiology

Background:

  • Ischemic stress and protein kinase C (PKC) receptor stimuli offer cardiac protection against ischemia-reperfusion injury.
  • Exogenous calcium (Ca2+) pretreatment provides PKC-mediated protection in animal models.
  • The efficacy of Ca2+ preconditioning in human myocardium and its underlying PKC mechanism remain unclear.

Purpose of the Study:

  • To investigate whether Ca2+ preconditioning confers postischemic functional protection in human myocardium.
  • To determine if this protection is mediated by a protein kinase C (PKC)-dependent mechanism.

Main Methods:

  • Human atrial trabeculae were subjected to simulated ischemia (45 minutes) and reperfusion (120 minutes).
  • Trabeculae received either exogenous CaCl2 (Ca2+ preconditioning) or vehicle before ischemia.

Related Experiment Videos

  • Protein kinase C (PKC) inhibition was applied concurrently with Ca2+ preconditioning in some groups.

    • Main Results:

    • Ischemia-reperfusion significantly reduced postischemic developed force in human atrial trabeculae.
    • Ca2+ preconditioning significantly protected human myocardium against ischemia-reperfusion injury compared to controls.
    • Inhibition of protein kinase C (PKC) abolished the protective effects of Ca2+ preconditioning.

    Conclusions:

    • Ca2+ preconditioning is a potent strategy for inducing protein kinase C (PKC)-mediated protection in human myocardium.
    • This study demonstrates the clinical relevance of Ca2+ preconditioning for mitigating ischemia-reperfusion injury in humans.