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Related Experiment Videos

Activation-induced NK cell death triggered by CD2 stimulation

H Ida1, P Anderson

  • 1Division of Rheumatology and Immunology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

European Journal of Immunology
|May 16, 1998
PubMed
Summary

The CD2 molecule on T cells and natural killer (NK) cells can trigger cell death in cytokine-primed NK cells. This rapid apoptosis, induced by anti-CD2 antibodies, highlights a common signaling pathway in activated lymphocytes.

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • T cells and natural killer (NK) cells express CD2, a molecule involved in cell activation.
  • CD2 ligation typically induces T cell proliferation, requiring co-expression of CD3:TCR, while NK cell proliferation requires FcgammaRIII.

Purpose of the Study:

  • To investigate if CD2 ligation can induce activation-induced cell death in IL-2-primed NK cells.
  • To explore the signaling pathways involved in CD2-mediated NK cell apoptosis.

Main Methods:

  • Utilized anti-CD2 monoclonal antibodies (mAbs) to stimulate freshly isolated and IL-2-primed NK cells.
  • Assessed apoptosis through chromatin condensation, DNA fragmentation, and caspase-3 cleavage.
  • Investigated the role of TNF-alpha and Fas ligand by using neutralizing antibodies.

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Main Results:

  • Anti-CD2 mAbs induced rapid apoptosis in IL-2-primed NK cells, characterized by chromatin condensation, DNA fragmentation, and caspase-3 activation.
  • CD2-induced apoptosis occurred within 90 minutes and was independent of TNF-alpha or Fas ligand.
  • Unlike T cell death, a single CD2 epitope was sufficient to trigger NK cell apoptosis.

Conclusions:

  • CD2 ligation can trigger rapid activation-induced cell death in cytokine-primed NK cells.
  • This process involves a signaling cascade potentially shared with other activation receptors like CD16 and CD94 in primed lymphocytes.