Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Neurotrophic factors decrease the release of creatine kinase and prostaglandin E2 from metabolically stressed muscle

J D Lian1, M al-Jumah, V Cwik

  • 1Mackenzie Health Science Centre, University of Alberta School of Medicine, Edmonton, Canada.

Neuromuscular Disorders : NMD
|May 5, 1998
PubMed
Summary

Neurotrophic factors like ciliary neurotrophic factor and insulin-like growth factor 1 show potential in protecting muscle cells from oxidative stress and damage.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Consensus recommendations for diagnosis and treatment of Multiple Sclerosis: 2023 revision of the MENACTRIMS guidelines.

Multiple sclerosis and related disorders·2024
Same author

Consensus recommendations for the diagnosis and treatment of multiple sclerosis: 2019 revisions to the MENACTRIMS guidelines.

Multiple sclerosis and related disorders·2019
Same author

Consensus recommendations for the diagnosis and treatment of multiple sclerosis: the Middle East North Africa Committee for Treatment and Research In Multiple Sclerosis (MENACTRIMS).

Current medical research and opinion·2015
Same author

HLA-B50 polymorphism in the Saudi population.

International journal of immunogenetics·2013
Same author

Consensus guidelines for the diagnosis and treatment of multiple sclerosis.

Current medical research and opinion·2013
Same author

Public attitude towards biomedical research at outpatient clinics of King Abdulaziz Medical City, Riyadh, Saudi Arabia.

Eastern Mediterranean health journal = La revue de sante de la Mediterranee orientale = al-Majallah al-sihhiyah li-sharq al-mutawassit·2011

Area of Science:

  • Biochemistry
  • Cell Biology
  • Neuroscience

Background:

  • Oxidative stress is a significant factor in muscle cell damage.
  • Creatine kinase (CK) and prostaglandin E2 (PGE2) are established biomarkers for muscle injury.

Purpose of the Study:

  • To investigate the cell-protective effects of specific neurotrophic factors against oxidative stress in an in vitro muscle model.
  • To evaluate the potential therapeutic applications of these factors in muscle diseases.

Main Methods:

  • An in vitro model of muscle oxidative stress was established using rat muscle exposed to 2,4-dinitrophenol.
  • The release of creatine kinase (CK) and prostaglandin E2 (PGE2) was measured as an indicator of cell damage.
  • The effects of ciliary neurotrophic factor (CNTF), brain-derived neurotrophic factor (BDNF), and insulin-like growth factor 1 (IGF-1) on cell damage markers were assessed.

Related Experiment Videos

Main Results:

  • Exposure to 2,4-dinitrophenol induced the release of CK and PGE2, confirming muscle cell damage.
  • CNTF, BDNF, and IGF-1 significantly prevented the release of both CK and PGE2.
  • These findings suggest a protective role for the investigated neurotrophic factors.

Conclusions:

  • Ciliary neurotrophic factor, brain-derived neurotrophic factor, and insulin-like growth factor 1 demonstrate cell-protective properties in an oxidative stress model.
  • These neurotrophic factors may hold therapeutic potential for treating muscle diseases characterized by cell damage.