Related Experiment Videos
Diagnosis of dengue by using reverse transcriptase-polymerase chain reaction
M P Miagostovich1, F B dos Santos, E S de Araújo
1Departamento de Virologia, Instituto Oswaldo Cruz, RJ, Brasil. marizepm@gene.dbbm.fiocruz.br
Memorias Do Instituto Oswaldo Cruz
|May 5, 1998
Abstract:
A rapid identification of dengue viruses from clinical samples by using a nested reverse transcriptase-polymerase chain reaction (RT-PCR) procedure was carried out for diagnostic and epidemiological purposes. RT-PCR identified DEN-1 and DEN-2 viruses in 41% (41/100) of previously confirmed cases and provided an accurate confirmation of DHF in four fatal cases. RT-PCR was also useful for detecting and typing dengue viruses in suspected cases, allowing a rapid identification of new serotypes in endemic areas.