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Diagnosis of dengue by using reverse transcriptase-polymerase chain reaction
M P Miagostovich1, F B dos Santos, E S de Araújo
1Departamento de Virologia, Instituto Oswaldo Cruz, RJ, Brasil. marizepm@gene.dbbm.fiocruz.br
Memorias Do Instituto Oswaldo Cruz
|May 5, 1998
Summary
A novel nested reverse transcriptase-polymerase chain reaction (RT-PCR) method enables rapid identification and typing of dengue viruses from clinical samples for diagnostic and epidemiological use.
Area of Science:
- Virology
- Molecular Biology
- Infectious Diseases
Background:
- Dengue virus (DENV) poses a significant global health threat, necessitating rapid diagnostic tools.
- Accurate identification and serotyping are crucial for effective disease management and epidemiological surveillance.
Purpose of the Study:
- To develop and evaluate a nested reverse transcriptase-polymerase chain reaction (RT-PCR) for rapid dengue virus identification.
- To assess the utility of RT-PCR for serotyping dengue viruses in clinical samples and fatal cases.
Main Methods:
- A nested RT-PCR assay was designed for the detection and typing of dengue viruses.
- The assay was applied to clinical samples from confirmed and suspected dengue cases, including fatal cases of dengue hemorrhagic fever (DHF).
Main Results:
- The nested RT-PCR successfully identified Dengue serotypes 1 (DEN-1) and 2 (DEN-2) in 41% of previously confirmed cases.
- The method accurately confirmed DENV in four fatal DHF cases.
- RT-PCR facilitated the rapid detection and typing of dengue viruses in suspected cases, aiding in identifying new serotypes in endemic regions.
Conclusions:
- Nested RT-PCR is a valuable tool for the rapid and accurate diagnosis and epidemiological surveillance of dengue virus infections.
- This method supports timely clinical management and public health interventions in dengue-endemic areas.