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Updated: Aug 11, 2026

A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells
Published on: July 3, 2013
Report of a hitherto unreported sequence present and expressed in a cancer cell line
1Department of Biophysics, Molecular Biology and Genetics, University of Calcutta, India.
Abstract:
During the amplification of a 202 base pair (bp) fragment around the 61st codon of the N-ras gene, an extra band of about 150 bp in length was observed when genomic DNA from a human epidermoid carcinoma cell line was used as template. This fragment was cloned and sequenced. However, this sequence was not found in the databank. RT-PCR experiments indicated that the sequence is expressed in the cells about 8 h after serum induction. The relevant RNA hybridized to one strand of the sequence but not to the other.
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