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Related Experiment Videos

Nested insertions of short mobile sequences in Drosophila P elements

S Hagemann1, W J Miller, E Haring

  • 1Institut für Medizinische Biologie, AG Allgemeine Genetik, Universität Wien, Vienna, Austria.

Chromosoma
|May 6, 1998
PubMed
Summary

Researchers sequenced a P element from Drosophila ambigua, finding it non-functional due to mutations and containing an insertion sequence (IS-amb-P). This mobile element shows homology to others in the obscura group and is located at specific chromosomal sites.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Evolutionary Biology

Background:

  • P elements are mobile genetic elements found in Drosophila.
  • The T-type P element subfamily and insertion sequences (IS) are important in genome evolution.
  • Comparative genomics of Drosophila species reveals insights into mobile element dynamics.

Purpose of the Study:

  • To characterize a full-sized P element from Drosophila ambigua.
  • To investigate the structure, genomic location, and evolutionary relationships of this P element and associated insertion sequences.
  • To compare P element organization across different Drosophila species.

Main Methods:

  • Polymerase chain reaction (PCR) amplification and complete DNA sequencing of the P element.
  • Sequence comparison and homology analysis with known mobile elements.

Related Experiment Videos

  • In situ hybridization to determine chromosomal localization of P elements.
  • Gene mapping using the sik-hom gene as a marker for interspecific comparison.

    • Main Results:

    • A 3329 bp T-type P element was sequenced, featuring 33 bp inverted terminal repeats.
    • All four exons of the P element contained stop codons and frameshift mutations, rendering it non-functional.
    • A 687 bp insertion sequence (IS-amb-P) was identified within the P element and also exists as dispersed copies (IS-amb) in the D. ambigua genome.
    • P elements in D. ambigua were localized to two euchromatic sites on chromosomes A and E, similar to related species.
    • IS element insertions into P elements appear to have occurred independently in different Drosophila lineages.

    Conclusions:

    • The sequenced P element from D. ambigua is transcriptionally inactive due to internal mutations.
    • The IS-amb element represents a mobile sequence in D. ambigua with homology to other obscura group mobile elements.
    • The chromosomal distribution and nested insertion patterns suggest independent evolutionary events for P elements and IS elements in D. ambigua and related species.