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Related Experiment Videos

Biochemical speciation of enterococci causing human infections

V Gulati1, A Aggarwal, S Khanna

  • 1Department of Microbiology, Govt. Medical College, Amritsar.

Indian Journal of Medical Sciences
|May 6, 1998
PubMed
Summary

Enterococci are significant nosocomial pathogens. This study found most isolates were Enterococcus faecalis, with high sensitivity to ampicillin, though Enterococcus faecium showed greater drug resistance.

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Area of Science:

  • Microbiology
  • Infectious Diseases
  • Antimicrobial Resistance

Background:

  • Enterococci are increasingly recognized as critical nosocomial pathogens.
  • Accurate speciation and antibiotic sensitivity testing are crucial for effective treatment.

Purpose of the Study:

  • To determine the speciation of Enterococcus isolates from clinical samples.
  • To evaluate the antibiotic susceptibility patterns of these isolates.

Main Methods:

  • Physiological and biological characterization of 120 Enterococcus isolates.
  • Speciation using established microbiological methods.
  • Antibiogram analysis to assess antibiotic sensitivity.

Main Results:

  • 95% of isolates were identified as Enterococcus faecalis, and 5% as Enterococcus faecium.

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  • 83.3% of isolates demonstrated sensitivity to ampicillin.
  • A combination of penicillin and gentamicin showed enhanced efficacy.
  • Enterococcus faecium exhibited higher resistance to antibiotics compared to E. faecalis.

    • Conclusions:

    • Enterococcus faecalis is the predominant species in this clinical setting.
    • Ampicillin remains a viable treatment option for many Enterococcus infections.
    • Enterococcus faecium poses a greater challenge due to its inherent drug resistance, necessitating alternative therapeutic strategies.