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Related Experiment Videos

Momordins inhibit both AP-1 function and cell proliferation

D K Lee1, B Kim, S G Lee

  • 1Department of Chemistry, Seoul National University, Korea.

Anticancer Research
|May 6, 1998
PubMed
Summary

Natural plant extracts were screened for inhibitors of Jun/Fos protein-DNA interaction, a key in tumor promotion. Momordin I from Ampelopsis radix showed potent anticancer activity by blocking this interaction.

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Area of Science:

  • Molecular Biology
  • Pharmacology
  • Natural Product Chemistry

Background:

  • Jun/Fos activation transmits tumor-promoting signals to nuclear transcription.
  • Jun/Fos binding to the AP-1 site is essential for gene transcription and cancer progression.

Purpose of the Study:

  • To identify natural plant extracts that inhibit Jun/Fos function.
  • To investigate the anticancer potential of identified compounds.

Main Methods:

  • Screening of approximately 100 plant extracts for Jun/Fos inhibitors.
  • Isolation and structural identification of active compounds using chromatography.
  • Quantification of Jun/Fos-DNA interaction inhibition via liquid scintillation counting.
  • Assessment of cell cytotoxicity against human cancer cell lines.

Related Experiment Videos

  • In vivo testing of the methanol extract against murine colon cancer.

    • Main Results:

    • The methanol extract of Ampelopsis radix significantly reduced Jun/Fos-DNA interaction.
    • Oleanolic acid glycosides, momordin I, Id, and Ie, were identified as active principles.
    • Momordin I exhibited the strongest inhibitory activity (IC50: 22.8 µg/ml) and cytotoxicity (IC50: 7.28–16.05 µg/ml).
    • The methanol extract demonstrated anticancer activity in vivo, reducing tumor weight by 17.73%.

    Conclusions:

    • Momordin I's anticancer activity is likely mediated by its inhibition of protein-DNA interaction.
    • Ampelopsis radix and its constituents represent potential therapeutic agents for cancer treatment.