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Experimental observation of photochemically induced oxygen depletion in perfused cells undergoing photodynamic
T Schunck1, J Steibel, P Poulet
1Institut de Physique Biologique, UPRES A 7004 1173 du CNRS, Faculté de Médecine, Université Louis Pasteur, Strasbourg, France.
Anticancer Research
|May 6, 1998
Abstract:
This report presents a non invasive method for studying oxygen consumption during photodynamic therapy in cultured cells. The oxygen partial pressure of perfusion medium flowing through cells cultured on microcarrier beads, was investigated before, during and after treatment. Pheophorbide a was used as a sensitiser. Time-dependent measurements demonstrate photochemical oxygen depletion induced by photosensitized reactions, followed by an oxygen pressure increase were attributed to a reduction in the cells' metabolism.