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Microsatellite alterations in uterine leiomyomas

D French1, C Cermele, A M Lombardi

  • 1Dipartimento di Medicina Sperimentale e Patologia, Cattedra di Citopatologià, Università La Sapienza, Roma, Italy.

Anticancer Research
|May 6, 1998
PubMed
Summary

Microsatellite instability (MI) was investigated in uterine leiomyomas. Alterations in microsatellites were found in 30.4% of samples, suggesting a role in benign smooth muscle cell transformation.

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Area of Science:

  • Oncology
  • Genetics
  • Pathology

Background:

  • Microsatellite instability (MI) is implicated in various cancers, characterized by alterations in repetitive DNA sequences.
  • High rates of microsatellite alterations are observed in familial polyposis and sporadic cancers of the colon, stomach, breast, and endometrium.

Purpose of the Study:

  • To investigate the presence and significance of microsatellite alterations in uterine leiomyomas.
  • To determine if microsatellite instability plays a role in the development of benign smooth muscle tumors.

Main Methods:

  • DNA from 23 uterine leiomyoma samples (ages 24-65) was analyzed.
  • Seven specific microsatellite markers were examined using polymerase chain reaction (PCR) with nested amplification.
  • Samples were analyzed on denaturing acrylamide gels to detect alterations.

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Main Results:

  • Microsatellite alterations were detected in 7 out of 23 analyzed samples (30.4%).
  • The identified alterations involved specific microsatellite markers, indicating genetic instability.

Conclusions:

  • The recurrence of microsatellite alterations in uterine leiomyomas suggests their potential involvement in the benign transformation of smooth muscle cells.
  • These findings contribute to understanding the molecular mechanisms underlying uterine leiomyoma development.