Related Experiment Videos
Cytologic and immunoenzymatic findings in CSF from patients with tick-borne encephalitis
1Dr. Fran Mihaljević University Hospital for Infectious Diseases, University of Zagreb, Croatia.
Objective:
To evaluate the dynamics of cytologic changes in the cerebrospinal fluid (CSF) of patients with tick-borne encephalitis (TBE) and to determine the various forms of mononuclear cells by immunocytochemical methods.
Study Design:
To perform a cytologic analysis of 200 cells in stained sediment of CSF. To determine the population and subpopulation of mononuclear cells by using monoclonal antibodies.
Results:
Pleocytosis in CSF in patients with TBE during the first three days of illness was, on average, 570 cells per cubic millimeter with about 60% neutrophils. Mononuclear cells predominated from the fifth day of illness. Among lymphoid cells, 18% carried marker CD20 and 60% of them marker CD3. Among monocytoid cells, 66% carried marker CD32.
Conclusion:
CSF cytology findings reflect the immunologic and inflammatory changes in the CNS during TBE.