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A transient CD15 immunoreactive sling in the developing mouse cerebellum

K W Ashwell1, J K Mai

  • 1School of Anatomy, University of New South Wales, Australia.

International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience
|May 6, 1998
PubMed
Summary

A transient structure, the transverse cerebellar sling, marked by CD15 (3-fucosyl-N-acetyl-lactosamine) immunoreactivity, was identified in the developing mouse cerebellum. This sling appears to play a role in prenatal cerebellar morphogenesis.

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Immunohistochemistry

Background:

  • The developing cerebellum undergoes complex cellular migrations and structural organization.
  • Specific molecular markers are crucial for understanding developmental processes and identifying transient structures.

Purpose of the Study:

  • To investigate the distribution and characteristics of the 3-fucosyl-N-acetyl-lactosamine (FAL, CD15) epitope in the developing mouse cerebellum.
  • To identify and characterize a novel transient structure potentially involved in cerebellar morphogenesis.

Main Methods:

  • Immunohistochemistry was performed on paraffin sections of developing mouse cerebella.
  • CD15 immunoreactivity was analyzed at different developmental stages (E15, E17, P2).

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Main Results:

  • CD15 immunoreactivity initially appeared as a discrete bundle of processes beneath the external granular layer at E15.
  • By E17, these processes formed a sling-like structure encircling the posterior cerebellum, connecting germinal trigones.
  • By P2, this sling disappeared, with CD15 immunoreactivity becoming restricted to astrocytes in the cerebellar white matter.

Conclusions:

  • A novel transient structure, the transverse cerebellar sling, composed of CD15-immunoreactive processes, was identified in the developing mouse cerebellum.
  • The sling's unusual trajectory and transient nature suggest a role in prenatal cerebellar morphogenesis.
  • Despite its trajectory and association with the ventricular surface, the sling was not immunoreactive for S-100 or GFAP, indicating a potentially unique glial cell type or composition.