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Pseudotype formation with La Crosse virus glycoproteins
1Department of Microbiology, University of Pennsylvania Medical Center, Philadelphia 19104-6146, USA.
The Journal of General Virology
|May 6, 1998
Summary
Researchers created La Crosse virus pseudotypes to study virus entry. Incorporating specific glycoproteins made the pseudotypes resistant or sensitive to monoclonal antibodies, revealing insights into bunyavirus neutralization.
Area of Science:
- Virology
- Molecular Biology
- Immunology
Background:
- Pseudotype formation is a valuable method for studying virus entry and neutralization mechanisms.
- It enables the analysis of viral glycoprotein functions without requiring the creation of recombinant viral genomes.
Purpose of the Study:
- To develop a technique for analyzing glycoprotein mutations in bunyaviruses using pseudotypes.
- To investigate the role of specific glycoprotein variants in La Crosse virus neutralization and entry.
Main Methods:
- Preparation of La Crosse virus pseudotypes using recombinant vaccinia viruses.
- Cloning and incorporation of recombinant glycoproteins from monoclonal antibody (MAb)-resistant variants (V31 and V33) into pseudotypes.
- Assessment of pseudotype sensitivity to neutralization by specific MAbs (807-31 and 807-33).
Main Results:
- Pseudotypes incorporating V31 glycoproteins showed partial resistance to MAb 807-31.
- Incorporation of V31 glycoproteins into the V33 variant resulted in pseudotypes sensitive to MAb 807-33.
- Demonstrated a straightforward method for introducing glycoprotein mutations into bunyaviruses.
Conclusions:
- The developed pseudotype system allows for the functional analysis of bunyavirus glycoprotein mutations.
- This technique facilitates the study of virus neutralization and entry mechanisms.
- Provides insights into the specific roles of glycoprotein variants in antibody-mediated viral resistance.