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Lack of surface receptors not restriction-modification system determines F4 phage resistance in Streptococcus bovis
I Styriak1, P Pristas, P Javorský
1Institute of Animal Physiology, Slovak Academy of Sciences, Kosice, Slovakia. styriak@linux1.saske.sk
Abstract:
The resistance of Streptococcus bovis strain II/1, the producer of SbvI restriction endonuclease, to F4 phage infection was demonstrated by the double-agar-layer method. Despite the presence of restriction endonuclease SbvI which can cleave F4 phage DNA to numerous fragments in vitro, the evidence that adsorption inhibition is the most important defence mechanism in phage resistance of S. bovis II/1 strain was obtained by adhesion experiments in vivo. Electron microscopy of phage-host mixtures showed many phage particles on the bacterial surface of phage-sensitive S. bovis 47/3 control strain in comparison with no phage particles seen on S. bovis II/1 (phage-resistant) strain surface.