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Published on: June 11, 2012
The level of MAP kinase activity in the stomach stump in rats after subtotal gastrectomy
T Banasiewicz1, R Marciniak, P Majewski
1Clinic of Surgery, Karol Marcinkowski University School of Medical Sciences, Poznań, Poland.
Abstract:
MAPK (Mitogen-Activated Protein Kinase) is one of the elements of kinase cascades (MAPK, MEK-MAP kinase, kinase, Raf-1, Ras) regulating cellular proliferation and differentiation processes. It seems that the changes in its number and activity may be the factor having influence on carcinogenesis. In some human carcinomas a significant increase of its activity is observed, in others a decrease of its activity is described. Our research aimed at the evaluation of the dynamics of precancerous and cancerous changes in the stomach stump in rats after the experimental, partial stomach resection. Apart from histological and ultrastructural examination we also determined the activity of the sub-unit p42 MAP kinase. The material comprised segments of gastric mucosa of the stomach stump of 15 rats after subtotal gastrectomy. Part of the rats after the procedure were administered carcinogen orally (MNNG). On the histological and ultrastructural examination we used routine methods, the activity of MAP kinase was determined by western-blotting method with the use of IgG against MAPK p42, Santa Cruz #154). In 8 examined rats we observed the increase of MAP kinase activity. We established probable correlation (without statistical analysis, regarding miserly material) between the increase of MAPK activity and histological and ultrastructural changes. Among three cases diagnosed as adenoma tubulare in two we observed the increase of MAPK activity. A clear increase of this kinase was also present in the stomach stump of a rat, which was diagnosed as adenocarcinoma. On the basis of our research carried so far we think that the increase of the MAPK activity may be one of the causes of the neoplasm development. It seems important to obtain the confirmation of our results and to establish a possible usefulness of MAPK activity determination as a prognostic indicator in case of the neoplasm of stomach stump.
Insights
Increased Mitogen-Activated Protein Kinase (MAPK) activity may drive stomach stump cancer development. This study observed elevated MAPK levels in precancerous and cancerous stomach tissues in rats, suggesting its potential as a prognostic indicator.
Area of Science:
- Oncology
- Molecular Biology
- Gastroenterology
Background:
- Mitogen-Activated Protein Kinase (MAPK) pathways regulate cell proliferation and differentiation.
- Aberrant MAPK activity is implicated in various human carcinomas, with conflicting reports on increased or decreased activity.
- Stomach stump cancer, a post-surgical complication, requires better understanding of its carcinogenesis mechanisms.
Purpose of the Study:
- To investigate the role of MAPK activity in the development of precancerous and cancerous changes in rat stomach stumps after partial resection.
- To evaluate the correlation between MAPK activity and histological/ultrastructural alterations in gastric mucosa.
Main Methods:
- Experimental partial stomach resection in rats followed by oral carcinogen administration (MNNG).
- Histological and ultrastructural examination of gastric mucosa segments.
- Determination of p42 MAP kinase activity using Western blotting.
Main Results:
- Increased MAP kinase activity was observed in 8 out of 15 rats.
- A probable correlation was noted between elevated MAPK activity and observed histological/ultrastructural changes.
- MAPK activity was increased in cases of tubular adenoma and adenocarcinoma in the stomach stump.
Conclusions:
- Elevated MAPK activity may contribute to the development of stomach stump neoplasms.
- Further research is needed to confirm these findings and explore MAPK activity as a prognostic indicator for stomach stump cancer.
