Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A simple and sensitive ribonucleotide reductase assay

A Y Jong1, K Yu, B Zhou

  • 1Department of Pediatrics, Childrens Hospital of Los Angeles and the University of Southern California School of Medicine, 90027, USA. ajong%smtpgate@chlais.usc.edu

Journal of Biomedical Science
|June 17, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gene silencing of non-obese diabetic receptor family (NLRP3) protects against the sepsis-induced hyper-bile acidaemia in a rat model.

Clinical and experimental immunology·2014
Same author

The effects of substance P on pluripotent tendon cells: an in vitro and in vivo study.

Journal of musculoskeletal & neuronal interactions·2014
Same author

Effects of partial or total replacement of maize with alternative feed source on digestibility, growth performance, blood metabolites and economics in limousin crossbred cattle.

Asian-Australasian journal of animal sciences·2014
Same author

Measurement of direct CP violation parameters in B± → J/ψK± and B± → J/ψπ± decays with 10.4 fb-1 of Tevatron data.

Physical review letters·2014
Same author

Precision measurement of the top quark mass in lepton + jets final States.

Physical review letters·2014
Same author

Effect of calcitonin on anastrozole-induced bone pain during aromatase inhibitor therapy for breast cancer.

Genetics and molecular research : GMR·2014

Researchers developed a new, faster, and more sensitive assay for Ribonucleotide reductase (RR), an enzyme crucial for DNA synthesis and a target for cancer drugs like hydroxyurea.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Enzymology

Background:

  • Ribonucleotide reductase (RR) is vital for DNA synthesis.
  • Current RR assays are time-consuming and labor-intensive.
  • RR is a key target for cancer chemotherapeutics, such as hydroxyurea.

Purpose of the Study:

  • To develop a novel, simplified, and sensitive assay for Ribonucleotide reductase (RR) activity.
  • To overcome the limitations of existing RR enzymatic assays.

Main Methods:

  • A new assay couples RR activity detection to DNA polymerase reactions.
  • RNase is used to degrade endogenous RNA, enhancing detection.
  • Radioactive ribonucleotide diphosphate ([14C]CDP) is used as a substrate.

Related Experiment Videos

Main Results:

  • The novel assay demonstrated linear substrate incorporation over 30 minutes.
  • Activity was linear with the amount of cell extract used.
  • The assay showed specificity for RR, being inhibited by hydroxyurea and requiring Mg2+ and ATP.

Conclusions:

  • The new RR assay is simpler, faster, more sensitive, and less expensive than conventional methods.
  • It allows for simultaneous multi-sample analysis.
  • This method offers significant advantages over existing RR assays.