Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DerivFit: a program for rate equation parameter fitting using derivatives

R Michalski1, W Rode, A Leś

  • 1Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland.

Computers and Biomedical Research, an International Journal
|May 8, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Detection and identification of oxidants formed during •NO/O2•⁻ reaction: a multi-well plate CW-EPR spectroscopy combined with HPLC analyses.

Free radical research·2014
Same author

Bioequivalence study of 8 mg ondansetron film-coated tablets in healthy Caucasian volunteers.

Drug research·2013
Same author

Refined preparation of 1alpha,3-dipyrrolidino-androsta-3,5-diene-17-one, a key intermediate in the exemestane synthesis.

Die Pharmazie·2012
Same author

Isolation of a homogeneous preparation of human thymidylate synthetase from HeLa cells.

Biochemical pharmacology·2010
Same author

Dihalide and pseudohalide radical anions as oxidizing agents in nonaqueous solvents.

The journal of physical chemistry. A·2009
Same author

Binding and repression of translation of the cognate mRNA by Trichinella spiralis thymidylate synthase differ from the corresponding interactions of the human enzyme.

The Biochemical journal·2007

This study introduces DerivFit, a C program for enzyme kinetics. It accurately fits kinetic parameters using ordinary differential equations and advanced algorithms for experimental data analysis.

Area of Science:

  • Biochemistry
  • Computational Chemistry
  • Enzyme Kinetics

Background:

  • Enzyme kinetics research requires accurate parameter fitting.
  • Existing methods may lack efficiency or generality.

Purpose of the Study:

  • To present DerivFit, a novel C program for fitting enzymatic rate equation parameters.
  • To provide a robust tool for analyzing enzyme reaction schemes described by ODEs.

Main Methods:

  • Utilizes ordinary differential equations (ODEs) to model reaction schemes.
  • Employs minimization algorithms (Gradient, Newton, Marquardt) to fit kinetic parameters.
  • Calculates gradients by solving additional ODEs for enhanced accuracy.

Main Results:

Related Experiment Videos

  • Successfully fitted kinetic parameters for enzymatic systems.
  • Demonstrated application to complex scenarios like slow tight-binding inhibition.
  • The program accurately predicts theoretical progress curves from experimental data.

    • Conclusions:

    • DerivFit offers an efficient and accurate method for enzyme kinetics parameterization.
    • The program's ODE-based approach and gradient calculation enhance its utility.
    • Applicable to a range of enzymatic systems, including challenging inhibition models.