Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nucleotide-dependent structural changes in dimeric NCD molecules complexed to microtubules

K Hirose1, R A Cross, L A Amos

  • 1National Institute for Advanced Interdisciplinary Research, Tsukuba 305, Japan.

Journal of Molecular Biology
|June 10, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mechanism of enhanced lipid peroxidation in the liver of Long-Evans cinnamon (LEC) rats.

Archives of toxicology·2000
Same author

Introduction of 6-formylcytidine into a Myb binding sequence.

Nucleosides & nucleotides·2000
Same author

Functional recovery after coronary artery bypass grafting in patients with severe left ventricular dysfunction and preserved myocardial viability in the left anterior descending arterial territory as assessed by thallium-201 myocardial perfusion imaging.

Japanese circulation journal·1999
Same author

Dynamic Ca2+ signalling in rat arterial smooth muscle cells under the control of local renin-angiotensin system.

The Journal of physiology·1999
Same author

Anthropogenic radionuclides in seawater of the Far Eastern Seas.

The Science of the total environment·1999
Same author

Utility of laboratory testing in autoimmune inner ear disease.

The Laryngoscope·1999
Same journal

BindRNAgen: Protein-binding RNA sequence generation using latent diffusion models.

Journal of molecular biology·2026
Same journal

Structural basis of HSP90C, a highly active chloroplastic HSP90 chaperone from Arabidopsis thaliana.

Journal of molecular biology·2026
Same journal

Clinical inflammasome biomarkers: Progress and prospects.

Journal of molecular biology·2026
Same journal

Biologically Relevant, Cationic Residues in Human Rhinovirus Stabilize Capsid-Bound RNA Duplexes, and Restrict Capsid Flexibility.

Journal of molecular biology·2026
Same journal

Cryo-EM structures of phage T4 infection intermediate.

Journal of molecular biology·2026
Same journal

A classic fold with a twist: Structural architecture of Dhillonvirus phage Bas18.

Journal of molecular biology·2026
See all related articles

Motor proteins like kinesin-like protein ncd bind microtubules using one head, with the other tethered. Their structural changes during ATP turnover do not drive directional movement, suggesting dimer arrangement dictates directionality.

Area of Science:

  • Molecular motor proteins
  • Structural biology
  • Cellular transport

Background:

  • Kinesin-like protein ncd motors are crucial for intracellular transport.
  • Understanding their nucleotide-bound states is key to elucidating motor function.

Purpose of the Study:

  • To visualize and analyze different nucleotide-associated states of the ncd motor domain.
  • To understand how these states influence motor-microtubule interactions and head positioning.

Main Methods:

  • Cryoelectron microscopy
  • Helical image reconstruction
  • Analysis of dimeric ncd constructs (NDelta295-700) in ADP, AMP.PNP, and rigor states.

Main Results:

  • Single-headed attachment to microtubules observed across all analyzed states.

Related Experiment Videos

  • Differences in attached head-tubulin interaction and tethered head positioning were identified.
  • The ATP-like (AMP.PNP) state showed the free head tilting towards the plus end.

    • Conclusions:

    • Conformational changes in the bound head do not directly guide the free head's position.
    • The relative orientation of ncd dimer heads is a primary determinant of motor directionality.
    • Tethered heads consistently point towards the minus end, supporting the dimer arrangement hypothesis.