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Crossregulation between En-2 and Wnt-1 in chick tectal development
S Sugiyama1, J Funahashi, J Kitajewski
1Department of Molecular Neurobiology, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan.
Development, Growth & Differentiation
|May 8, 1998
Summary
Chick embryo optic tectum development involves En-2 and Wnt-1 signaling. Their crossregulation is confirmed in the diencephalon, potentially mimicking early optic tectum development.
Area of Science:
- Developmental biology
- Neuroscience
- Molecular biology
Background:
- En-1, En-2, and Wnt-1 are crucial signals for chick optic tectum development.
- Drosophila homologs, engrailed (En) and wingless (Wnt), exhibit crossregulation.
Purpose of the Study:
- To investigate the crossregulation between En-2 and Wnt-1 in chick optic tectum development.
- To determine if this crossregulation is conserved in the diencephalon.
Main Methods:
- Overexpression of En-2 using replication-competent retroviral vectors.
- Ectopic Wnt-1 introduction via mesencephalon transplantation or Wnt-1 producing cell implantation.
Main Results:
- En-2 overexpression induced ectopic Wnt-1 expression in the diencephalon.
- Wnt-1 introduction led to ectopic En-2 induction at the tel-diencephalic border.
- Demonstrated crossregulation between En-2 and Wnt-1 in the chick brain.
Conclusions:
- Crossregulation between En-2 and Wnt-1 is preserved in chick optic tectum development.
- Diencephalic crossregulation of En-2 and Wnt-1 may model early optic tectum development signals.