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Related Experiment Videos

Leukocyte adhesion molecule expression during intense resistance exercise

M P Miles1, S K Leach, W J Kraemer

  • 1Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park 16802, USA.

Journal of Applied Physiology (Bethesda, Md. : 1985)
|June 6, 1998
PubMed
Summary

Exercise alters immune cell distribution, with lymphocyte L-selectin expression decreasing post-workout. Very late antigen-4 (VLA-4) integrin levels remained stable on lymphocytes during exercise.

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Area of Science:

  • Immunology
  • Exercise Physiology

Background:

  • Adhesion molecules like L-selectin and very late antigen-4 (VLA-4) integrin play roles in immune cell trafficking.
  • Understanding how these molecules change during exercise is crucial for comprehending immune responses.

Purpose of the Study:

  • To investigate the impact of exercise on the expression of L-selectin and VLA-4 integrin on immune cells.
  • To determine if these adhesion molecules influence cell-type specific redistribution during physical exertion.

Main Methods:

  • Women subjects performed resistance exercise (10-repetition maximum squats).
  • Flow cytometry was used to measure L-selectin and VLA-4 integrin expression on neutrophils and lymphocytes pre- and post-exercise.
  • Analysis included lymphocyte subsets.

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Main Results:

  • Neutrophil and lymphocyte concentrations increased significantly post-exercise.
  • L-selectin expression on neutrophils remained unchanged.
  • A significant decrease in L-selectin positive T cells, natural killer cells, and B cells was observed post-exercise.
  • VLA-4 integrin expression was consistently high on lymphocytes before and after exercise.

Conclusions:

  • Exercise induces significant changes in lymphocyte populations and L-selectin expression.
  • The observed decrease in L-selectin positive cells suggests potential shedding, selective entry of negative subsets, or selective removal of positive subsets.
  • VLA-4 integrin appears less affected by this type of exercise in lymphocytes.