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Mutational analysis of the phosphate-binding loop of Rhizobium meliloti DctD, a sigma54-dependent activator
1Department of Microbiology, University of Georgia, Athens, Georgia 30602, USA.
Abstract:
The phosphate-binding loop of sigma54-dependent activators is thought to participate in ATP binding and/or hydrolysis. Alanine substitutions at positions 3, 4, 6, 7, and 8 of this motif in Rhizobium meliloti DctD disrupted transcriptional activation and ATP hydrolysis. Interestingly, substitution of alanine at position 7 also affected DNA binding.
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