Fatty acids and fatty aldehydes of buffalo seminal plasma and sperm lipid

Analysis of the fatty acids of total and neutral lipids, glycolipids, phospholipids and gangliosides of buffalo spermatozoa and seminal plasma showed that there were high levels of polyunsaturated acids. Neutral lipids were the richest in polyunsaturated acids (55% in spermatozoa and 61% in seminal plasma). The major saturated acid of all the principal classes was stearic acid and the major unsaturated acid was docosahexaenoic acid (22:6omega3) except in the neutral lipids in which it was arachidonic acid (20:4omega6). The major aldehyde was palmitaldehyde (16:0) in buffalo sperm lipids and docosanal (22:0) in seminal plasma. More than 50% of the total aldehydes was contributed by aldehydes with a chain length greater than 18 carbon atoms.