[Establishment and characterization of primitive neuroectodermal tumor cell line (UTO-EW1)]

New human primitive neuroectodermal tumor cell line, UTO-EW1 was established from the humeral bone tumor of a 25-year-old male patient. UTO-EW1 cells were found to be small round shaped and grew floating in the medium, or found to form monolayers in a polygonal shaped cells. The modal clomosomal number of the cells fell in a hypo-triploid range with several clonal clomosomal aberrations, including del(22) (q12). Neuron-specific enolase was positive by stained immunohistochemically. This cell line may be useful for basic studies and treatment of PNET.