Related Experiment Videos
Hemodialysis stimulates hepatocyte growth factor release
T Rampino1, C Libetta, W De Simone
1Department of Internal Medicine and Nephrology, University and Policlinico San Matteo, Pavia, Italy.
Kidney International
|May 9, 1998
Summary
Dialysis significantly increases serum hepatocyte growth factor (HGF) levels and stimulates HGF release from peripheral blood mononuclear cells (PBMC). This suggests dialysis activates factors that promote HGF production and release.
Area of Science:
- Nephrology
- Cell Biology
- Biochemistry
Background:
- Hepatocyte growth factor (HGF) plays a crucial role in tissue repair and regeneration.
- The impact of dialysis on HGF levels and production remains incompletely understood.
Purpose of the Study:
- To investigate the effect of hemodialysis on serum HGF concentration.
- To examine HGF production by peripheral blood mononuclear cells (PBMC) during dialysis.
- To assess the biological activity of serum from dialysis patients.
Main Methods:
- Serum HGF levels and PBMC HGF production were measured in 26 dialysis patients using various dialyzers (CU, PMMA, CAM).
- Blood samples were collected at different time points during dialysis (T0, T15, T240).
- Immunoblotting was used to analyze HGF forms, and cell proliferation assays were performed.
Main Results:
- Dialysis induced a significant increase in serum HGF levels, peaking at 15 minutes.
- PBMC collected during dialysis showed enhanced in vitro HGF release.
- Serum from dialysis patients stimulated tubular cell proliferation and HGF release from other cell types.
Conclusions:
- Hemodialysis leads to a substantial elevation in circulating HGF.
- Dialysis triggers the release of factors that stimulate HGF production and release.
- Dialysis-activated PBMCs contribute to increased HGF levels.