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Related Experiment Videos

Expression and function of the type 3 complement receptor in tissues of the developing mouse

D A Hughes1, S Gordon

  • 1Sir William Dunn School of Pathology, University of Oxford, United Kingdom.

Journal of Immunology (Baltimore, Md. : 1950)
|May 9, 1998
PubMed
Summary

This study reveals that complement receptor 3 (CR3) plays a crucial role in neonatal macrophage adhesion, spreading, and phagocytosis. However, CR3-independent pathways are also vital for monocyte migration during development.

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Area of Science:

  • Immunology
  • Developmental Biology
  • Cell Biology

Background:

  • Leukocyte integrins, like CR3, are essential for adult macrophage function.
  • Limited understanding exists regarding immune cell integrin expression and function during embryonic development.

Purpose of the Study:

  • To map the expression patterns of complement receptor 3 (CR3) in developing mice.
  • To assess CR3's functional role in neonatal macrophage adhesion, spreading, and phagocytosis.
  • To investigate CR3's involvement in inflammatory and constitutive cell migration during development.

Main Methods:

  • Utilized anti-CR3 monoclonal antibody (mAb) 5C6 to study CR3 function.
  • Examined CR3 expression in relation to F4/80 glycoprotein in developing tissues.
  • Assessed neonatal macrophage functions (adhesion, spreading, phagocytosis) in vitro.

Related Experiment Videos

  • Investigated in vivo migration of myelomonocytic cells to inflammatory stimuli.

    • Main Results:

    • CR3 expression on monocytes appears early, while its expression on stellate and resident tissue macrophages varies temporally and spatially.
    • Neonatal macrophages utilize CR3-dependent mechanisms for adhesion, spreading, and phagocytosis, with a CR3-independent system also contributing to spreading.
    • CR3 blockade significantly impacted early inflammatory migration of myelomonocytic cells in vivo.
    • Monocyte-derived resident macrophage populations developed normally despite antibody transfer, indicating CR3-independent migration pathways.

    Conclusions:

    • CR3 is functionally important for neonatal macrophage effector functions and early inflammatory cell migration.
    • Developmental monocyte migration and differentiation into resident macrophages involve CR3-dependent and CR3-independent mechanisms.
    • CR3 expression patterns change significantly from embryonic development to adulthood, with specific resident macrophage populations retaining CR3.