Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Intermediates in adenovirus assembly

B Edvardsson, E Everitt, H Jörnvall

    Journal of Virology
    |August 1, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Coping and self-image in patients with symptoms attributed to indoor environment.

    Archives of environmental & occupational health·2013
    Same author

    Chorea associated with vitamin B12 deficiency.

    European journal of neurology·2011
    Same author

    Virion glycosylation governs integrity and infectivity of infectious pancreatic necrosis virus.

    Journal of fish diseases·2011
    Same author

    Giant cell arteritis and bilateral peroneal nerve palsy.

    Scandinavian journal of rheumatology·2010
    Same author

    Pre-orgasmic headache and cervical carotid artery dissection.

    European journal of neurology·2009
    Same author

    Medical and social prognoses of non-specific building-related symptoms (Sick Building Syndrome): a follow-up study of patients previously referred to hospital.

    International archives of occupational and environmental health·2007

    This study defines three adenovirus assembly intermediates: nuclear, young, and mature virions. These stages reveal the sequential processing of viral proteins during adenovirus assembly.

    Area of Science:

    • Virology
    • Molecular Biology
    • Cell Biology

    Background:

    • Adenovirus assembly involves distinct intermediate stages.
    • Understanding these intermediates is crucial for deciphering viral replication mechanisms.

    Purpose of the Study:

    • To characterize the properties and polypeptide composition of three adenovirus assembly intermediates.
    • To elucidate the sequential processing of viral proteins during adenovirus assembly.

    Main Methods:

    • Differential gradient centrifugation (Ficoll and CsCl) to separate intermediates.
    • Pulse-chase labeling and tryptic peptide mapping to analyze polypeptide processing.
    • Cell fractionation to localize intermediates within cellular compartments.

    Main Results:

    Related Experiment Videos

    • Nuclear intermediates are fragile, heterogeneous, and contain precursor polypeptides (pVI, pVII).
    • Young virions are stable, nuclear, and contain both precursor and cleaved polypeptides.
    • Mature virions are cytoplasmic and contain final mature polypeptides.
    • Polypeptide pVI is the precursor to VI; 26K polypeptide partially accounts for VIII.

    Conclusions:

    • The three defined intermediates represent sequential stages in adenovirus assembly.
    • Protein processing and localization changes mark the progression through these assembly stages.
    • This provides a framework for understanding adenovirus morphogenesis.