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Isolation and identification of CGRP C-terminal fragments in the rat spinal cord
P Le Grevès1, K Andersson, J Silberring
1Department of Pharmaceutical Biosciences, University of Uppsala, Sweden. pierrelegreves@farmbio.uu.se
Abstract:
In this study, we have used a liquid chromatography micropurification system in combination with fast atom bombardment mass spectrometry (FAB-MS) and N-terminal sequencing to characterize 3 calcitonin gene-related peptide (CGRP) immunoreactivities present in the rat spinal cord. Full-length CGRP contributed to approximately 68% of the total immunoreactive material, while approximately 23% consisted of 2 C-terminal fragments, CGRP(18-37) and CGRP(19-37). Synthetic C-terminal fragments of CGRP, e.g. CGRP(19-37), have been shown to antagonize CGRP effects in vitro. We show that such fragments exist in relatively substantial amounts in the rat spinal cord.