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Selective intraarterial gene delivery into a canine meningioma
A E Chauvet1, P P Kesava, C S Goh
1Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, USA.
Object:
The goal of this study was to evaluate gene delivery to a benign brain tumor.
Methods:
A recombinant adenovirus vector bearing the Escherichia coli beta-galactosidase reporter gene was selectively injected into the vascular supply of a spontaneously occurring canine olfactory groove meningioma. The tumor and a small amount of peritumoral brain tissue were removed 5 days after viral injection and stained with X-Gal to assess gene delivery. The authors noted significant beta-galactosidase gene expression by the tumor, but not by surrounding brain tissue. No obvious viral-related cytotoxicity was noted.
Conclusions:
The authors found that meningiomas can be successfully transduced by adenovirus vectors by using endovascular techniques.