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Soluble L-selectin concentration in bronchoalveolar lavage fluid obtained from infants who develop chronic lung
S Kotecha1, M Silverman, R J Shaw
1Department of Paediatrics, Hammersmith Hospital, London.
Insights
Neutrophil adhesion molecules, like soluble L-selectin, remain elevated in infants who develop chronic lung disease (CLD), suggesting persistent leukocyte activity contributes to CLD pathogenesis.
Area of Science:
- Neonatal immunology
- Pulmonary medicine
- Cellular biology
Background:
- Chronic lung disease (CLD) is a significantcomplication in premature infants.
- Understanding the inflammatory mechanisms underlying CLD is crucial for developing effective treatments.
Purpose of the Study:
- To investigate changes in neutrophil adhesion molecule expression and release in bronchoalveolar lavage fluid (BAL) of infants who develop CLD.
- To determine the role of leukocyte traffic in the pathogenesis of CLD.
Main Methods:
- Bronchoalveolar lavage (BAL) fluid was collected from 37 infants, categorized into those who developed CLD, those with respiratory distress syndrome (RDS), and healthy controls.
- Expression of soluble L-selectin (sL-selectin) and CD11b/CD18 on neutrophils was analyzed in BAL fluid.
Main Results:
- Infants who developed CLD showed persistently increased levels of sL-selectin in BAL fluid compared to RDS and control groups.
- Neutrophil CD11b/CD18 expression increased over time, peaking around 17 days of age in infants who developed CLD.
Conclusions:
- Persistent elevation of neutrophil adhesion molecules suggests ongoing leukocyte activity in infants developing CLD.
- Leukocyte traffic may play a critical role in the development and progression of chronic lung disease in neonates.
Aims:
To explore the changes in neutrophil adhesion molecule expression and release into bronchoalveolar lavage fluid (BAL) obtained from infants who developed chronic lung disease (CLD).
Methods:
BAL fluid was obtained from 37 infants: 18 (median gestation 26 weeks, birthweight 835 g) who developed CLD, 12 (29 weeks, 1345 g) with respiratory distress syndrome (RDS) and seven control infants (33 weeks, 2190 g).
Results:
Soluble L-selectin (sL-selectin) in BAL fluid from the CLD and non-CLD groups was similar immediately after birth, but in infants who subsequently developed CLD, sL-selectin remained persistently increased (at day 7: CLD 42.6 vs RDS 6.0 ng/ml, p < 0.05; CLD vs controls 1.5 ng/ml; p < 0.05). CD11b/CD18 expression on neutrophils obtained by BAL increased with time to reach a maximum at 17 days of age in infants who developed CLD.
Conclusions:
These results suggest that leucocyte traffic persists in infants who develop CLD and may have an important part to play in the pathogenesis of CLD.