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Updated: Jul 16, 2026

mRNA Interactome Capture from Plant Protoplasts
Published on: July 28, 2017
Processing of vertebrate box C/D small nucleolar RNAs in plant cells
D J Leader1, G P Clark, J Boag
1Department of Cell and Molecular Genetics, Scottish Crop Research Institute, Dundee, UK.
Abstract:
The recent isolation of a number of plant box C/D small nucleolar (sno)RNAs demonstrates the conservation in plants of sequence and structural elements of processed box C/D snoRNAs. Boxes C and D, and terminal inverted repeats are known to be essential for accumulation and processing in vertebrates and yeast. Processing of vertebrate box C/D snoRNAs was examined by expression of various mouse hsc70 intron 5-U14 constructs in tobacco protoplasts. Full-length U14 and internally deleted U14 accumulated in the plant cells. Human U3 and U8 fragments, consistent with processing to internal box C/C' sequences, also accumulated in the plant cells. The similarity of processing behaviour of the vertebrate box C/D constructs in tobacco protoplasts and Xenopus oocytes suggests the mechanism of processing, involving recognition and association of proteins, is conserved in plants.
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