Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Retroviral vector targeting human cells via c-Kit-stem cell factor interaction

T Yajima1, T Kanda, K Yoshiike

  • 1The Heart Institute of Japan, Tokyo Women's Medical College.

Human Gene Therapy
|May 15, 1998
PubMed
Summary

Researchers developed a targeted retroviral vector for gene therapy, utilizing a modified envelope protein to bind c-Kit receptors on stem cells. This advancement aids in developing in vivo stem cell gene therapy strategies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Human papillomavirus type 16 minor capsid protein l2 N-terminal region containing a common neutralization epitope binds to the cell surface and enters the cytoplasm.

Journal of virology·2001
Same author

Nasal immunization of mice with peptide having a cross-neutralization epitope on minor capsid protein L2 of human papillomavirus type 16 elicit systemic and mucosal antibodies.

Vaccine·2001
Same author

Enhancer-promoter activity of human papillomavirus type 16 long control regions isolated from cell lines SiHa and CaSki and cervical cancer biopsies.

Japanese journal of cancer research : Gann·2000
Same author

Adeno-associated virus type 2 nonstructural protein Rep78 suppresses translation in vitro.

Virology·1999
Same author

DNA vaccination of mice with plasmid expressing human papillomavirus 6 major capsid protein L1 elicits type-specific antibodies neutralizing pseudovirions constructed in vitro.

Journal of medical virology·1999
Same author

Common neutralization epitope in minor capsid protein L2 of human papillomavirus types 16 and 6.

Journal of virology·1999

Area of Science:

  • Molecular Biology
  • Gene Therapy
  • Virology

Background:

  • Hematopoietic stem cell (HSC) gene transfer is crucial for in vivo stem cell gene therapy.
  • Retroviral vectors are commonly used for gene delivery.
  • Targeting specific cell receptors can enhance gene therapy efficacy.

Purpose of the Study:

  • To engineer a recombinant retroviral vector capable of targeting cells expressing the c-Kit receptor.
  • To investigate the potential of ligand-receptor interactions for targeted gene delivery to HSCs.

Main Methods:

  • Modification of the ecotropic Moloney murine leukemia virus envelope protein by incorporating murine stem cell factor (mSCF).
  • Pseudotyping retroviral particles with the chimeric envelope protein.
  • Transduction assays on cells expressing murine and human c-Kit, with and without chloroquine, and on fibronectin-coated dishes.

Related Experiment Videos

Main Results:

  • The chimeric envelope protein was efficiently processed and incorporated into viral particles.
  • Virions preferentially bound to c-Kit-positive cells but required chloroquine or specific cell culture conditions for transduction.
  • Recombinant mSCF in the medium reduced transduction efficiency, indicating reliance on the mSCF-c-Kit interaction.

Conclusions:

  • A recombinant retroviral vector was successfully engineered to target c-Kit-expressing cells.
  • The vector utilizes the mSCF-c-Kit interaction for targeted gene delivery.
  • This targeted vector shows promise for in vivo stem cell gene therapy applications.