Related Experiment Videos
A high-resolution microsatellite map of the mouse genome
M Rhodes1, R Straw, S Fernando
1United Kingdom Human Genome Mapping Project (HGMP) Resource Centre, Hinxton CB10 1RQ, UK.
Genome Research
|May 20, 1998
Summary
The European Collaborative Interspecific Backcross (EUCIB) resource enabled the creation of a high-resolution mouse genome genetic map. This map, featuring 3368 microsatellites, enhances physical mapping efforts for the mouse genome.
Area of Science:
- Genomics
- Genetic mapping
Background:
- The European Collaborative Interspecific Backcross (EUCIB) resource was established for detailed mouse genome genetic mapping.
- A large Mus spretus/C57BL/6 backcross with 982 progeny provides high genetic resolution (0.3 cM).
Purpose of the Study:
- To develop a genome-wide, high-resolution genetic map of the mouse genome using the EUCIB resource.
- To facilitate the construction of a robust physical map of the mouse genome.
Main Methods:
- Utilized the EUCIB mapping resource, comprising 982 progeny from a Mus spretus/C57BL/6 backcross.
- Incorporated 3368 microsatellites into the genetic map.
Main Results:
- Developed a genome-wide high-resolution genetic map with 3368 microsatellites.
- Microsatellites are organized into 2302 distinct bins, averaging 1.46 markers per bin.
- Achieved an average bin separation of 0.61 cM, offering precise genomic localization.
Conclusions:
- The generated high-resolution genetic map is a valuable tool for mouse genome research.
- This map will significantly aid in the development of a comprehensive physical map of the mouse genome.