Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Progression through the spliceosome cycle requires Prp38p function for U4/U6 snRNA dissociation

J Xie1, K Beickman, E Otte

  • 1T.H. Morgan School of Biological Sciences, University of Kentucky, Lexington, KY 40506-0225, USA.

The EMBO Journal
|June 10, 1998
PubMed
Summary

Prp38p is crucial for spliceosome activation, enabling pre-mRNA splicing. Without this protein, spliceosomes assemble but fail to perform essential catalytic steps, halting RNA processing.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Analysis of factors related to human soil-transmitted nematode infections in Chongqing City].

Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control·2019
Same author

[Comparative study of differentiation potential of mesenchymal stem cells derived from orofacial system into vascular endothelial cells].

Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences·2019
Same author

[Neck gout stone: a case report].

Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery·2019
Same author

Broncho-vaxom alleviates persistent allergic rhinitis in patients by improving Th1/Th2 cytokine balance of nasal mucosa.

Rhinology·2019
Same author

Perioperative myocardial injury after noncardiac surgery: An easily ignored and challenging problem.

International journal of cardiology·2019
Same author

Cerebral blood flow abnormalities in neuropsychiatric systemic lupus erythematosus.

Lupus·2019

Area of Science:

  • Molecular Biology
  • RNA Processing
  • Protein Function

Background:

  • Pre-mRNA splicing is a complex process involving the spliceosome.
  • Prp38p is known to be essential for the initial pre-mRNA cleavage step.
  • The precise role of Prp38p in spliceosome maturation remained unclear.

Purpose of the Study:

  • To elucidate the specific function of Prp38p in spliceosome maturation and catalytic activation.
  • To determine the localization and necessity of Prp38p within spliceosomal complexes.

Main Methods:

  • Investigating the role of Prp38p in spliceosome assembly and function in vitro and in vivo.
  • Analyzing the composition of spliceosomes lacking Prp38p activity, focusing on snRNP interactions.
  • Assessing the impact of Prp38p on RNA cleavage and snRNA dynamics during splicing.

Related Experiment Videos

Main Results:

  • Prp38p is a unique component of the U4/U6.U5 tri-small nuclear ribonucleoprotein (snRNP) particle.
  • Prp38p is essential for a late-stage spliceosome maturation step, not for initial assembly.
  • Prp38p-deficient spliceosomes are catalytically impaired and retain U4 snRNA, preventing activation.

Conclusions:

  • Prp38p is required for conformational changes that activate the spliceosome's catalytic function.
  • Prp38p facilitates the release of U4 snRNA, a critical step for pre-mRNA cleavage.
  • This study identifies Prp38p as a key regulator of spliceosome activation, distinct from its role in initial cleavage.