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[Effect of 1,8-ANS on structural characteristics of cardiomyocytes sarcolemma]
O I Lebed'1, V V Zhirnov, A I Grinevich
1Institute of Pharmacology and Toxicology, Academy of Medical Sciences of Ukraine.
Abstract:
The effect of sufan on structure properties of the rat cardiomyocyte sarcolemma was investigated. Fluorescence methods involving the probing with 1-anilino-8-naphthalene-sulfonate or pyrene and the self-fluorescence of membrane proteins was used. Effect of sufan on the cardiomyocyte sarcolemma structure has been determined to provoke the conformational disturbances. These are the compaction of membrane proteins and translocation of their tryptophanyls from the surface to the interior of macromolecules. This process was accompanied by the increase of a protein plunge into the lipid matrix. Sufan induces a decrease of the microviscosity without a change of the polarity index in hydrophobic region of the lipid bilayer. These mechanisms have been supposed to be significant for the exhibition of sufan cardioactive properties.