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Nucleoside-nucleotide mixture increases bone marrow cell number and small intestinal RNA content in protein-deficient
K Yamauchi1, A A Adjei, C K Ameho
1Research Center of Comprehensive Medicine, Department of Nutrition, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan.
Abstract:
The aim of this study was to determine if intraperitoneal administration of a nucleoside-nucleotide mixture would affect small intestinal morphology, bone marrow cell number, and DNA content in protein-deficient mice subjected to acute bacterial infection. Mice were randomized into two groups and orally fed protein-free diet or nucleotide-free 20% casein diet for 10 d. The mice in each group were divided into two subgroups and intraperitoneally administered 0.35 mL saline or nucleoside-nucleotide mixture (17.5 mL/kg body weight) for 10 d. On day 10, one subgroup from each major dietary group was either inoculated intravenously with methicillin-resistant Staphylococcus aureus or saline. Three days later, small intestinal morphology, bone marrow cell number, and DNA content were evaluated in infected and noninfected mice. Protein-deficiency in association with infection significantly (P < 0.05) reduced body weight, small intestinal weight, crypt depth, villous height, and wall thickness. All dietary groups exhibited similar small intestinal DNA and protein contents (protein:DNA ratio, RNA:DNA ratio) at 3 d postinfection. However, small intestinal RNA content in the infected protein-free dietary group administered nucleoside-nucleotide mixture was higher (P < 0.05) and tended to be higher relative to the infected nucleotide-free 20% casein group administered nucleoside-nucleotide mixture compared with the rest of the groups. In the infected protein-free dietary group administered nucleoside-nucleotide mixture, bone marrow cell number and bone marrow DNA content were higher (P < 0.05) relative to the infected protein-free dietary group, nucleotide-free 20% casein diet administered saline, or nucleoside-nucleotide mixture, respectively. We conclude that intraperitoneal administration of nucleoside-nucleotide mixture may stimulate bone marrow cell proliferation, DNA content, and small intestinal RNA content during periods of relative deficiency such as protein-deficiency in combination with infection.
Insights
Nucleotide supplementation may improve gut and bone marrow health in protein-deficient, infected mice. This study shows a nucleoside-nucleotide mixture enhances small intestinal RNA and bone marrow cell proliferation during infection and protein deficiency.
Area of Science:
- Biochemistry
- Nutrition
- Immunology
Background:
- Protein deficiency and bacterial infection impair host physiology.
- Nucleotides play crucial roles in cellular processes and immune function.
Purpose of the Study:
- To investigate the effects of a nucleoside-nucleotide mixture on small intestinal morphology, bone marrow cell number, and DNA content.
- To assess these effects in protein-deficient mice with acute bacterial infection.
Main Methods:
- Mice were fed protein-free or nucleotide-free diets and administered saline or a nucleoside-nucleotide mixture.
- Infection was induced using methicillin-resistant Staphylococcus aureus.
- Small intestinal morphology, bone marrow cell number, and DNA content were analyzed post-infection.
Main Results:
- Protein deficiency combined with infection significantly reduced body weight and small intestinal parameters.
- Nucleoside-nucleotide mixture administration increased small intestinal RNA content in infected protein-deficient mice.
- Bone marrow cell number and DNA content were elevated in infected protein-deficient mice receiving the nucleoside-nucleotide mixture.
Conclusions:
- Intraperitoneal nucleoside-nucleotide mixture administration can stimulate bone marrow cell proliferation and DNA content.
- The mixture may also enhance small intestinal RNA content during combined protein deficiency and infection.
- Nucleotide supplementation shows potential therapeutic benefits in compromised nutritional and infectious states.