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Collection and Extraction of Saliva DNA for Next Generation Sequencing
Published on: August 27, 2014
Technical note: improved DNA extraction from ancient bones using silica-based spin columns
1Department of Anthropology, McMaster University, Hamilton, Ontario, Canada.
American Journal of Physical Anthropology
|May 19, 1998
Abstract:
We describe a simple method for extracting polymerase chain reaction-amplifiable DNA from ancient bones without the use of organic solvents. Bone powders are digested with proteinase K, and the DNA is purified directly using silica-based spin columns (QIAquick3, QIAGEN). The efficiency of this protocol is demonstrated using human bone samples ranging in age from 15 to 5,000 years old.
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