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Synthesis and properties of oligonucleotide derivatives containing a hydrophobic fluorescent dye at the 2'-position
T Mitsui1, H Hayashi, K Yamana
1Department of Applied Chemistry, Himeji Institute of Technology, Japan.
Nucleic Acids Symposium Series
|January 1, 1997
Abstract:
The oligonucleotides containing 2'-(6-dimethylamino-2-naphthamide)uridine [U(DAN)] at the different position were synthesized by the fully automated solid phase phosphoramidite methods. The UV melting profiles for the U(DAN)-modified oligonucleotides with complementary DNA exhibited sigmoidal curves whose shapes were similar to those for the unmodified duplexes.